Asian Journal of Research in Chemistry

P. Jayaseelan ¹, S. Prasad ¹, R. Rajavel ¹

Affiliations
1 Department of Chemistry, Periyar University, Salem-11, Tamil Nadu, IN

Abstract

The tetradentate Schiff base ligand was prepared by condensation of 2-aminobenzaldehyde with o-phenylenediamine. The synthesized complex has been studied by using cyclic voltammetry and DNA cleavage studies. The resulting voltammogram consists of a single quasi-reversible one electron transfer attribute to couple [Cu(II)L]/[Cu(I)L]. Trends in cathodic peak potential (Epc) values are observed which can be correlated with electron effects of Schiff base ligand, changes with basis of liquid groups are determinant for electrochemical trends. The interaction of Cu(II) complex with Calf Thymus DNA has been studied by using absorption, viscosity and cyclic voltammetry. Cyclic voltammetry studies reveal that the complex prefer to bind to DNA in Cu(II) rather than (I) oxidation state.

Keywords

Schiff Base, Copper(II), 2-Aminobenzaldehyde, Voltammetry, DNA Cleavage Studies.

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Ismail ¹, R. Rajavel ¹, M. Ganesh ¹, M. Jagadeeswaran ¹, K. Srinivasan ¹, J. Valarmathi ¹, T. Sivakumar ¹

Affiliations
1 Nandha College of Pharmacy, Division of Pharmaceutical Analysis, Koorapalayam Pirivu, Erode-638052, Tamil Nadu, IN

Abstract

A simple, precise, accurate and rapid HPLC method has been developed, and validated for the determination of Aspirin, Atorvastatin and Pioglitazone simultaneously, in combined dosage form. Acetonitrile and phosphate buffer with pH 3.5 (40%:60% v/v) is used as the mobile phase, 261nm is the detection wavelength for this study. The applicability of the method for simultaneous determination of Aspirin, Atorvastatin and Pioglitazone was verified by the determination of these compounds in marketed tablets. Results of the analysis were validated statistically, and by recovery studies (98.29-101.12%). The recovery and RSD values within the limits given in ICH guide lines method developments indicates that the suitability of proposed methods for the routine determination of these compounds in tablets. The validation parameters: linearity (r>0.996), sensitivity (LOD (1.5-0.7×10^-4 mg ml^-1 and LOQ%x10^-4-1.05×10^-4), accuracy (recoveries:98.29-101.12%)and reproducibility were found to satisfactory. The proposed method can be successfully used to determine the drug contents of marketed formulation.

Keywords

RP-HPLC, Aspirin, Pioglitazone, Atorvastatin.

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